ABSTRACT
Objective To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides (T. satureioides) and Mentha pulegium (M. pulegium), and to study their chemical composition. Methods The aerial parts of T. satureioides and M. pulegium (leaves and stems) were hydro-distillated using a Clevenger-type apparatus and essential oils were analyzed and identified by gas chromatography-mass spectrometry. Antimycobacterial screening of essential oils was performed on the basis of the inhibition zone diameter by disc diffusion method against two mycobacterial strains whereas the minimal inhibitory concentration and minimal bactericidal concentration were determined by using the micro-dilution method. Results Chemical analysis of their aerial part's essential oils gave as major compounds, borneol (34.26%), carvacrol (31.21%) and thymol (3.71%) for T. satureioides and R(+)-pulegone (75.48%), carvone (6.66%) and dihydrocarvone (4.64%) for M. pulegium. Thereafter their antimycobacterial effect evaluation, using the micro-dilution method, indicated that minimal inhibitory concentration values of T. satureioides essential oil ranged from 0.062% to 0.015% (v/v) and from 0.125% to 0.031% (v/v) for M. pulegium respectively against Mycobacterium aurum and Mycobacterium smegmatis. Conclusions It is clearly evident from the results obtained that the Moroccan medicinal plants have great potential to be used as anti-tuberculosis agents. These findings may help scientists to undertake several research projects to discover useful natural product as new anti-tuberculosis drug.
ABSTRACT
Objective: To investigate antioxidant activity, total phenolic and flavonoid content of ethanol (E) and ethyl acetate (A) extracts of Inula viscosa aerial parts selected from three regions of Morocco (Imouzzer, Sefrou and Taounate). Methods: Antioxidants properties were measured by three different test systems of assay namely free radical scavenging activities against 2,2-diphenyl-1-picrylhydrazyl, total antioxidant capacity and iron-reducing capacity. Total phenolic content was measured by Folin- Ciocalteu reagent. Results: All the extracts showed significant antioxidant activities and contained important levels of phenols. The ethanol extract (0.3 mg/mL) from Sefrou showed the greatest antioxidant capacity in the three systems of assay, which was probably due to its high content of polyphenols (274.39±6.94) mg gallic acid equivalent/g dry extract. Total flavonoid content was found equal for all extracts. Conclusions: Our results of antioxidant assays were justified and partially supported the popular usage of the tested plants. The high antioxidant activity found in the plant from Sefrou and its great biomass in this region suggested that Inula viscosa is a good source of natural antioxidants compounds which might have benefits for health.